منابع مشابه
Effects of thrombin, PAR-1 activating peptide and a PAR-1 antagonist on umbilical artery resistance in vitro
BACKGROUND The non-thrombotic effects of thrombin in cardiovascular tissues, as mediated via the protease activated receptors (PARs), and particularly PAR-1, have been the focus of much recent research. The aims of this study were to evaluate the effects of thrombin, a specific PAR-1 activating peptide (PAR1-AP), and a PAR-1 antagonist on human umbilical artery tone in vitro. METHODS Human um...
متن کاملCardiovascular responses mediated by protease-activated receptor-2 (PAR-2) and thrombin receptor (PAR-1) are distinguished in mice deficient in PAR-2 or PAR-1.
We developed mice deficient in protease-activated receptor-2 (PAR-2) or PAR-1 to explore the pathophysiological functions of these receptors. In this report, we evaluated mean arterial pressure and heart rate (HR) changes in response to PAR-1 or PAR-2 activation in anesthetized wild-type (WT), PAR-1-deficient (PAR-1(-/-)), and PAR-2-deficient (PAR-2(-/-)) mice. In WT mice, TFLLRNPNDK, a PAR-1 s...
متن کاملReceptor binding and agonist efficacy: new insights from mutants of the thrombin protease-activated receptor-1 (PAR-1).
The article by Blackhart et al. (2000), describing the differential activation of mutated thrombin protease-activated receptor-1 (PAR-1) either by the proteolytically revealed tethered ligand or by soluble receptor-activating peptides, adds yet another challenge to the theoretical understanding of receptor mechanisms. It is useful to view these new findings with the perspective offered by class...
متن کاملProtein kinase Cβ regulates heterologous desensitization of thrombin receptor (PAR-1) in endothelial cells.
We studied the effects of protein kinase C (PKC) activation on endothelial cell surface expression and function of the proteolytically activated thrombin receptor 1 (PAR-1). Cell surface PAR-1 expression was assessed by immunofluorescence (using anti-PAR-1 monoclonal antibody), and receptor activation was assessed by measuring increases in cytosolic Ca2+ concentration in human dermal microvascu...
متن کاملTime course of recovery of endothelial cell surface thrombin receptor (PAR-1) expression.
We studied dynamics of cell surface expression of proteolytically activated thrombin receptor (PAR-1) in human pulmonary artery endothelial cells (HPAEC). PAR-1 activation was measured by changes in cytosolic calcium concentration ([Ca2+]i) and HPAEC retraction response (determined by real-time transendothelial monolayer electrical resistance). [Ca2+]iincrease in response to thrombin was abolis...
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ژورنال
عنوان ژورنال: Japanese Journal of Thrombosis and Hemostasis
سال: 2012
ISSN: 1880-8808,0915-7441
DOI: 10.2491/jjsth.23.47